Visualisation of sentinel lymph node with indium-based near infrared emitting Quantum Dots in a murine metastatic breast cancer model.
Identifieur interne : 000939 ( Main/Exploration ); précédent : 000938; suivant : 000940Visualisation of sentinel lymph node with indium-based near infrared emitting Quantum Dots in a murine metastatic breast cancer model.
Auteurs : RBID : pubmed:22952979English descriptors
- KwdEn :
- Animals, Cell Death, Cell Line, Tumor, Disease Models, Animal, Erythrocytes (cytology), Female, Fibroblasts (cytology), Fluorescence, Hemolysis, Humans, Indium (diagnostic use), Lymph Nodes (pathology), Mammary Neoplasms, Animal (diagnosis), Mammary Neoplasms, Animal (pathology), Mice, Mice, Inbred BALB C, Neoplasm Metastasis, Quantum Dots, Spectroscopy, Near-Infrared, Time Factors, Tissue Distribution, Toxicity Tests.
- MESH :
- chemical , diagnostic use : Indium.
- cytology : Erythrocytes, Fibroblasts.
- diagnosis : Mammary Neoplasms, Animal.
- pathology : Lymph Nodes, Mammary Neoplasms, Animal.
- Animals, Cell Death, Cell Line, Tumor, Disease Models, Animal, Female, Fluorescence, Hemolysis, Humans, Mice, Mice, Inbred BALB C, Neoplasm Metastasis, Quantum Dots, Spectroscopy, Near-Infrared, Time Factors, Tissue Distribution, Toxicity Tests.
Abstract
Due to its non-invasiveness, high temporal resolution and lower cost, fluorescence imaging is an interesting alternative to the current method (blue dye and radiocolloid) of sentinel lymph node (SLN) mapping in breast cancer. Near-infrared (NIR) emitting cadmium-based Quantum Dots (QDs) could be used for this purpose; however, their wide application is limited because of the toxicity of heavy metals composing the core. Our recent work demonstrated that indium-based QDs exhibit a weak acute local toxicity in vivo compared to their cadmium-based counterparts. In the present study we confirmed the weak toxicity of CuInS(2)/ZnS QDs in different in vitro models. Further in vivo studies in healthy mice showed that In-based QDs could be visualised in SLN in a few minutes after administration with a progressive increase in fluorescence until 8 h. The quantity of indium was assessed in selected organs and tissues by inductively coupled plasma - mass spectroscopy (ICP-MS) as a function of post-injection time. QD levels decrease rapidly at the injection point in the first hours after administration with a parallel increase in the lymph nodes and to a lesser extent in the liver and spleen. In addition, we observed that 3.5% of the injected indium dose was excreted in faeces in the first 4 days, with only trace quantities in the urine. Metastatic spread to the lymph nodes may hamper its visualisation. Therefore, we further performed non-invasive fluorescence measurement of QDs in SLN in tumour-bearing mice. Metastatic status was assessed by immunohistology and molecular techniques and revealed the utmost metastatic invasion of 36% of SLN. Fluorescence signal was the same irrespective of SLN status. Thus, near-infrared emitting cadmium-free QDs could be an excellent SLN tracer.
DOI: 10.1371/journal.pone.0044433
PubMed: 22952979
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Visualisation of sentinel lymph node with indium-based near infrared emitting Quantum Dots in a murine metastatic breast cancer model.</title><author><name sortKey="Helle, Marion" uniqKey="Helle M">Marion Helle</name><affiliation wicri:level="1"><nlm:affiliation>Université de Lorraine, Centre de Recherche en Automatique de Nancy (CRAN), UMR 7039, Vandoeuvre-lès-Nancy, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université de Lorraine, Centre de Recherche en Automatique de Nancy (CRAN), UMR 7039, Vandoeuvre-lès-Nancy</wicri:regionArea><placeName><region type="région" nuts="2">Lorraine</region><settlement type="city">Vandœuvre-lès-Nancy</settlement><settlement type="city" wicri:auto="agglo">Nancy</settlement></placeName></affiliation></author><author><name sortKey="Cassette, Elsa" uniqKey="Cassette E">Elsa Cassette</name></author><author><name sortKey="Bezdetnaya, Lina" uniqKey="Bezdetnaya L">Lina Bezdetnaya</name></author><author><name sortKey="Pons, Thomas" uniqKey="Pons T">Thomas Pons</name></author><author><name sortKey="Leroux, Agnes" uniqKey="Leroux A">Agnès Leroux</name></author><author><name sortKey="Plenat, Francois" uniqKey="Plenat F">François Plénat</name></author><author><name sortKey="Guillemin, Francois" uniqKey="Guillemin F">François Guillemin</name></author><author><name sortKey="Dubertret, Benoit" uniqKey="Dubertret B">Benoît Dubertret</name></author><author><name sortKey="Marchal, Frederic" uniqKey="Marchal F">Frédéric Marchal</name></author></titleStmt><publicationStmt><date when="2012">2012</date><idno type="doi">10.1371/journal.pone.0044433</idno><idno type="RBID">pubmed:22952979</idno><idno type="pmid">22952979</idno><idno type="wicri:Area/Main/Corpus">000A84</idno><idno type="wicri:Area/Main/Curation">000A84</idno><idno type="wicri:Area/Main/Exploration">000939</idno></publicationStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cell Death</term><term>Cell Line, Tumor</term><term>Disease Models, Animal</term><term>Erythrocytes (cytology)</term><term>Female</term><term>Fibroblasts (cytology)</term><term>Fluorescence</term><term>Hemolysis</term><term>Humans</term><term>Indium (diagnostic use)</term><term>Lymph Nodes (pathology)</term><term>Mammary Neoplasms, Animal (diagnosis)</term><term>Mammary Neoplasms, Animal (pathology)</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Neoplasm Metastasis</term><term>Quantum Dots</term><term>Spectroscopy, Near-Infrared</term><term>Time Factors</term><term>Tissue Distribution</term><term>Toxicity Tests</term></keywords><keywords scheme="MESH" type="chemical" qualifier="diagnostic use" xml:lang="en"><term>Indium</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Erythrocytes</term><term>Fibroblasts</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Mammary Neoplasms, Animal</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Lymph Nodes</term><term>Mammary Neoplasms, Animal</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Death</term><term>Cell Line, Tumor</term><term>Disease Models, Animal</term><term>Female</term><term>Fluorescence</term><term>Hemolysis</term><term>Humans</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Neoplasm Metastasis</term><term>Quantum Dots</term><term>Spectroscopy, Near-Infrared</term><term>Time Factors</term><term>Tissue Distribution</term><term>Toxicity Tests</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Due to its non-invasiveness, high temporal resolution and lower cost, fluorescence imaging is an interesting alternative to the current method (blue dye and radiocolloid) of sentinel lymph node (SLN) mapping in breast cancer. Near-infrared (NIR) emitting cadmium-based Quantum Dots (QDs) could be used for this purpose; however, their wide application is limited because of the toxicity of heavy metals composing the core. Our recent work demonstrated that indium-based QDs exhibit a weak acute local toxicity in vivo compared to their cadmium-based counterparts. In the present study we confirmed the weak toxicity of CuInS(2)/ZnS QDs in different in vitro models. Further in vivo studies in healthy mice showed that In-based QDs could be visualised in SLN in a few minutes after administration with a progressive increase in fluorescence until 8 h. The quantity of indium was assessed in selected organs and tissues by inductively coupled plasma - mass spectroscopy (ICP-MS) as a function of post-injection time. QD levels decrease rapidly at the injection point in the first hours after administration with a parallel increase in the lymph nodes and to a lesser extent in the liver and spleen. In addition, we observed that 3.5% of the injected indium dose was excreted in faeces in the first 4 days, with only trace quantities in the urine. Metastatic spread to the lymph nodes may hamper its visualisation. Therefore, we further performed non-invasive fluorescence measurement of QDs in SLN in tumour-bearing mice. Metastatic status was assessed by immunohistology and molecular techniques and revealed the utmost metastatic invasion of 36% of SLN. Fluorescence signal was the same irrespective of SLN status. Thus, near-infrared emitting cadmium-free QDs could be an excellent SLN tracer.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">22952979</PMID><DateCreated><Year>2012</Year><Month>09</Month><Day>06</Day></DateCreated><DateCompleted><Year>2013</Year><Month>02</Month><Day>07</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1932-6203</ISSN><JournalIssue CitedMedium="Internet"><Volume>7</Volume><Issue>8</Issue><PubDate><Year>2012</Year></PubDate></JournalIssue><Title>PloS one</Title><ISOAbbreviation>PLoS ONE</ISOAbbreviation></Journal><ArticleTitle>Visualisation of sentinel lymph node with indium-based near infrared emitting Quantum Dots in a murine metastatic breast cancer model.</ArticleTitle><Pagination><MedlinePgn>e44433</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1371/journal.pone.0044433</ELocationID><Abstract><AbstractText>Due to its non-invasiveness, high temporal resolution and lower cost, fluorescence imaging is an interesting alternative to the current method (blue dye and radiocolloid) of sentinel lymph node (SLN) mapping in breast cancer. Near-infrared (NIR) emitting cadmium-based Quantum Dots (QDs) could be used for this purpose; however, their wide application is limited because of the toxicity of heavy metals composing the core. Our recent work demonstrated that indium-based QDs exhibit a weak acute local toxicity in vivo compared to their cadmium-based counterparts. In the present study we confirmed the weak toxicity of CuInS(2)/ZnS QDs in different in vitro models. Further in vivo studies in healthy mice showed that In-based QDs could be visualised in SLN in a few minutes after administration with a progressive increase in fluorescence until 8 h. The quantity of indium was assessed in selected organs and tissues by inductively coupled plasma - mass spectroscopy (ICP-MS) as a function of post-injection time. QD levels decrease rapidly at the injection point in the first hours after administration with a parallel increase in the lymph nodes and to a lesser extent in the liver and spleen. In addition, we observed that 3.5% of the injected indium dose was excreted in faeces in the first 4 days, with only trace quantities in the urine. Metastatic spread to the lymph nodes may hamper its visualisation. Therefore, we further performed non-invasive fluorescence measurement of QDs in SLN in tumour-bearing mice. Metastatic status was assessed by immunohistology and molecular techniques and revealed the utmost metastatic invasion of 36% of SLN. Fluorescence signal was the same irrespective of SLN status. Thus, near-infrared emitting cadmium-free QDs could be an excellent SLN tracer.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Helle</LastName><ForeName>Marion</ForeName><Initials>M</Initials><Affiliation>Université de Lorraine, Centre de Recherche en Automatique de Nancy (CRAN), UMR 7039, Vandoeuvre-lès-Nancy, France.</Affiliation></Author><Author ValidYN="Y"><LastName>Cassette</LastName><ForeName>Elsa</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Bezdetnaya</LastName><ForeName>Lina</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Pons</LastName><ForeName>Thomas</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Leroux</LastName><ForeName>Agnès</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Plénat</LastName><ForeName>François</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Guillemin</LastName><ForeName>François</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Dubertret</LastName><ForeName>Benoît</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Marchal</LastName><ForeName>Frédéric</ForeName><Initials>F</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType>Journal Article</PublicationType><PublicationType>Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>08</Month><Day>30</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>PLoS One</MedlineTA><NlmUniqueID>101285081</NlmUniqueID><ISSNLinking>1932-6203</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>045A6V3VFX</RegistryNumber><NameOfSubstance>Indium</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="Cites"><RefSource>J Clin Oncol. 2000 Jul;18(13):2560-6</RefSource><PMID Version="1">10893287</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Ann Surg Oncol. 2011 Sep;18(9):2483-91</RefSource><PMID Version="1">21360250</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Natl Toxicol Program Tech Rep Ser. 2001 Jul;(499):7-340</RefSource><PMID Version="1">12087422</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Breast J. 2002 Jul-Aug;8(4):192-8</RefSource><PMID Version="1">12100110</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Ann Oncol. 2002 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MajorTopicYN="N">cytology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Fluorescence</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Hemolysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Indium</DescriptorName><QualifierName MajorTopicYN="Y">diagnostic use</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Lymph Nodes</DescriptorName><QualifierName MajorTopicYN="Y">pathology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Mammary Neoplasms, Animal</DescriptorName><QualifierName MajorTopicYN="Y">diagnosis</QualifierName><QualifierName MajorTopicYN="N">pathology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Mice, Inbred BALB C</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Neoplasm Metastasis</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="Y">Quantum Dots</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="Y">Spectroscopy, Near-Infrared</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Time Factors</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Tissue Distribution</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Toxicity Tests</DescriptorName></MeshHeading></MeshHeadingList><OtherID Source="NLM">PMC3431369</OtherID></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2012</Year><Month>3</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2012</Year><Month>8</Month><Day>3</Day></PubMedPubDate><PubMedPubDate 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